Protein_Domain

Part:BBa_M10046:Design

Designed by: Dirk van Swaay   Group: Berkeley BioE140L - S09   (2009-05-08)

{˂CBK˃} Cellulose Binding Knottin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Combine 10pmol each of Odvs003,Odvs004,Odvs005,Odvs006
Run 55 program
Use product as template for:

PCR Odvs003/Odvs006 on assembly product (160bp, EcoRI/BamHI)
Product is pBca9495AK-M10046          {<CBK>}
--------------------------------------
Odvs003	Gene Synthesis of CBK basic part	CCATAGAATTCATGAGATCTGGTTCTGGTCCGACCCAGTCTCACTACGGTCAGTGCG
Odvs004	Gene Synthesis of CBK basic part	CGCAAACGGTCGGACCAGAGTAACCGATACCACCGCACTGACCGTAGTGAGACTG
Odvs005	Gene Synthesis of CBK basic part	CTCTGGTCCGACCGTTTGCGCTTCTGGTACCACCTGCCAGGTTCTGAACCCGTACTAC
Odvs006	Gene Synthesis of CBK basic part	GCTTAGGATCCACCAGAACCCAGGCACTGAGAGTAGTACGGGTTCAGAACCTGGC

Source

Source: synthetic.


References

<biblio>

  1. 1 pmid=2554967
  2. 3 pmid=8901566

</biblio>