Protein_Domain
Part:BBa_M10046:Design
Designed by: Dirk van Swaay Group: Berkeley BioE140L - S09 (2009-05-08)
{˂CBK˃} Cellulose Binding Knottin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Combine 10pmol each of Odvs003,Odvs004,Odvs005,Odvs006 Run 55 program Use product as template for: PCR Odvs003/Odvs006 on assembly product (160bp, EcoRI/BamHI) Product is pBca9495AK-M10046 {<CBK>} -------------------------------------- Odvs003 Gene Synthesis of CBK basic part CCATAGAATTCATGAGATCTGGTTCTGGTCCGACCCAGTCTCACTACGGTCAGTGCG Odvs004 Gene Synthesis of CBK basic part CGCAAACGGTCGGACCAGAGTAACCGATACCACCGCACTGACCGTAGTGAGACTG Odvs005 Gene Synthesis of CBK basic part CTCTGGTCCGACCGTTTGCGCTTCTGGTACCACCTGCCAGGTTCTGAACCCGTACTAC Odvs006 Gene Synthesis of CBK basic part GCTTAGGATCCACCAGAACCCAGGCACTGAGAGTAGTACGGGTTCAGAACCTGGC
Source
Source: synthetic.
References
<biblio>
- 1 pmid=2554967
- 3 pmid=8901566
</biblio>